Two-photon calcium imaging experiments to investigate the properties of neuronal microcircuits: the example of cerebellar network activity

Marialuisa Tognolina, Egidio D'Angelo
Abstract:
We have developed a custom-made two-photon microscope integrating a spatial light modulator that can modulate the phase of a laser beam and divide it into multiple diffraction-limited beamlets configurable in real time. We used this system to study cerebellar circuit activity and long-term synaptic plasticity expression by simultaneously acquiring stimulus-induced calcium signals from neurons located in different cerebellar layers.
Download:
IMEKO-TC2-2025-018.pdf
DOI:
10.21014/tc2-2025.018
Event details
IMEKO TC:
TC2
Event name:
IMEKO TC2 PhotoMet 2025
Title:

2025 IMEKO TC2 International Symposium on Modern Photonic Metrology

PhotoMet 2025 - Shaping the Future of Photonic Metrology

Place:
Modena, ITALY
Time:
01 September 2025 - 03 September 2025