Eventual limits of the current EU official method for evaluating milk adulteration of water buffalo dairy products and potential proteomic solutions |
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| Caira, S., Nicolai, M. A., Lilla, S., Calabrese, M. G., Pinto, G., Scaloni, A., Chianese, L., Addeo, F. |
- Abstract:
- The European reference method (ERM) identifies the fraudulent addition of bovine (B) milk in water buffalo (WB) milk/dairy products by concomitant isoelectric focusing (IEF) detection of B γ2- and γ3-CN fragments after plasminolysis. False positive results could occurr as the WB β-CN (f100- 209), also formed after plasminolysis of WB milk/dairy products comigrates with B γ2-CN. These ERM limitations were overcome by a phosphopeptides enrichment procedure followed by trypsinolysis and the elution of B β-CN(f1-25)4P and WB β-CN(f1-28)4P peptides identified as suitable specie/specific markers with a detection limit of 0.8% v/v.
- Keywords:
- gamma-2- and gamma-3-casein, peptide markers, milk, cheese, proteomics
- Download:
- IMEKO-TC23-2017-144.pdf
- DOI:
- -
- Event details
- IMEKO TC:
- TC23
- Event name:
- 3rd IMEKOFOODS Conference
- Title:
Metrology Promoting Standardization and Harmonization in Food and Nutrition
- Place:
- Thessaloniki, GREECE
- Time:
- 01 October 2017 - 04 October 2017